Journal: Current environmental health reports

Article Title: Adverse Maternal Metabolic Intrauterine Environment and Placental Epigenetics: Implications for Fetal Metabolic Programming

doi: 10.1007/s40572-018-0217-9

Figure Lengend Snippet: Global DNA methylation and epigenome-wide studies of gestational diabetes, maternal glucose and obesity

Article Snippet: Cardenas et al 2018 Ref. 65 , N= 448 French-Canadian Canada Gen3G Birth Cohort Sherbrooke, Quebec , Maternal glucose following 2h post-OGTT test (2nd trimester, wk 24–28) , 850K Infinium Methylation BeadChip EPIC Array Bisulfite DNA Pyrosequencing , Robust linear regression models adjusted for maternal age, BMI, parity, smoking during pregnancy, gestational age, sex and the first 10 principal components estimated from a reference-free cell-type heterogeneity method as proxy for cellular heterogeneity. P-values adjusted with FDR at 5% (q-value<0.05) Bisulfite DNA pyrosequencing for replication in another smaller population (N=65 to 108). , Seven CpG sites (q<0.05) associated with ↑ 1 mMol/L 2h-glucose postOGTT: TNFRSF1B (cg26189983) 1.22% ↑ methylation PDE4B (cg07734160, cg13866577, cg03442467, cg13349623) ↓ methylation (−0.58 to −1.16%) BLM (cg20254265) −0.63% ↓ methylation LDLR (cg08483713) −0.27% ↓ methylation. PDE4B methylation (cg03442467 gene body/TSS200) correlated with PDE4B ↑ expression (r=0.31; P=1.81×10 −3 ), TNFRSF1B methylation (cg26189983) correlated with expression (r= −0.24; P=0.013). LDLR methylation (cg08483713 gene body) associated with ↑ expression (r=0.32, P=9.62×10 −4 ).

Techniques: DNA Methylation Assay, Biomarker Discovery, Methylation, Control, Microarray, Expressing, Mass Spectrometry

Journal: Nature reviews. Endocrinology

Article Title: ‘Omics’ and Endocrine Disrupting Chemicals – New Paths Forward

doi: 10.1038/nrendo.2017.81

Figure Lengend Snippet: Strengths and limitations of omics technologies in EDC research

Article Snippet: Epigenomics , DNA Methylation , Pyrosequencing-Microarray; whole-genome sequencing , Examines the DNA methylome, ranging from gene specific areas to a microarray of about 850K sites to the entire DNA methylome (6) (7), which can impact gene expression , Can examine gene specific methylation and/or epigenome wide DNA methylation (up to 95%) , Tissue specific, so might not be the best representation if target tissue is not obtained.

Techniques: DNA Sequencing, Sequencing, Genome Wide, Microarray, Expressing, In Vitro, DNA Methylation Assay, Methylation, Chromatin Immunoprecipitation, Modification, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Genomic Sequencing